Gene expression analysis in zebrafish embryos Back to list of tests

Gene expression analysis in zebrafish embryos

Description:

Assessment of gene expression of selected genes (cyp1a, hsp or EDC-related and others) in Danio rerio embryo test; Various assays were developed such as GenDarT or MolDarT.

Reference:
Weil M. et al. (2009) Environ Toxicol Chem. 28(9):1970-8 / Muncke et al. 2007 Environ Toxicol 22, 185

Categorization of the assay/approach:

Status of the assay:

Application domain:

Categorization according to "exposure duration/effect":

Categorization according to combination of type of effect, endpoint measured and/or method used:

Categorization according to what "R" from the "3R" concept is this assay an alternative:

Details:

Other references:

Muncke Jane; Junghans Marion; Eggen Rik I. L. (2007) Testing estrogenicity of known and novel (xeno-) estrogens in the MolDarT using developing zebrafish (Danio rerio). ENVIRONMENTAL TOXICOLOGY 22 (2) 185-193 DOI: 10.1002/tox.20255 Published: APR 2007
 

Voelker Aquat Toxicol. 2007 Mar 30;81(4):355-64. Epub 2007 Jan 12.