Küster and Altenburger, Chemosphere. 2007 Jun;68(4):751-60. Epub 2007 Feb 9.
Categorization of the assay/approach:
Status of the assay:
Categorization according to "exposure duration/effect":
Categorization according to combination of type of effect, endpoint measured and/or method used:
Categorization according to what "R" from the "3R" concept is this assay an alternative:
Suborganismic effects were examined analysing the enzyme inhibition of cholinesterases and carboxylesterases, the enzyme inhibition analysis of whole homogenates of exposed embryos may be advantageous for toxicant screening (biomarker of exposure) and might be used to bridge the gap of sensitivity of the (48h old) zebrafish embryos to adult fish when exposed to anti-cholinesterase substances (biomarker of prospective effect).