Fish cell line – multiple cellular and biochemical endpoints Back to list of tests

Fish cell line – multiple cellular and biochemical endpoints

Description:

Several markers studied on fish cell line.

Reference:
Zurita et al. Toxicology in Vitro Volume 19, Issue 7, October 2005, Pages 879-886

Categorization of the assay/approach:

Status of the assay:

Application domain:

Categorization according to "exposure duration/effect":

Categorization according to combination of type of effect, endpoint measured and/or method used:

Categorization according to what "R" from the "3R" concept is this assay an alternative:

Details:

Cell morphology, total protein content, neutral red uptake, MTS metabolization, lysosomal function, succinate dehydrogenase activity, G6PDH activity, metallothionein levels and EROD activity were studied in the hepatoma fish cell line PLHC-1, derived from Poeciliopsis lucida (Zurita et al. Toxicology in Vitro Volume 19, Issue 7, October 2005, Pages 879-886.)


We treated rainbow trout RTG-2 cells with the polycyclic ketone tonalide (AHTN) at dilutions ranging from 3.5 to 500 ng/ml. The following parameters were monitored: intracellular ATP concentration (energy production), mitochondrial membrane potential (early apoptosis marker), cell viability (vital staining with DFP), quantitative expression of genes coding for the cytochrome P450 detoxifying enzymes CYP1A1 and CYP3A27, and of genes coding for the immunoregulatory peptides IL-1β, IL-8, TNFα, Cox-2 and TGF-β (Randelli et al. Toxicology in Vitro).

Time-dependent modulation of cyp1a gene transcription and EROD activity by musk xylene in PLHC-1 and RTG-2 fish cell lines (Della Torre et al. Toxicology in Vitro)

Fish hepatoma cell line PLHC-1: Cytotoxicity and interactions with cytochrome P450 1A (Thibaut and Cinta Porte,Toxicology in Vitro,Volume 22, Issue 5, August 2008, Pages 1128-1135)