Bird embryo hepatocyte in vitro assay Back to list of tests

Bird embryo hepatocyte in vitro assay

Description:

Effects on mRNA expression in bird (mostly chicken) hepatocytes

Reference:
Crump et al., Comp Biochem Physiol C Toxicol Pharmacol. 2011 Aug;154(2):129-34

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Status of the assay:

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Details:

Relative mRNA expression levels of genes involved in xenobiotic metabolism, thyroid hormon transport and lipid metabolism (mostly cytochrome P4501A)

other references: Hickley et al., Toxicol Sci. 2009 Oct;111(2):311-20, Hervé et al., Toxicol Sci. 2010 Feb;113(2):380-91, ...

Cultured CEH were prepared from day 19 embryos by collagenase digestion and filtration of pooled liver samples (n=50) as described previously (Kennedy et al., 1993; Head et al., 2006).