Crump et al., Comp Biochem Physiol C Toxicol Pharmacol. 2011 Aug;154(2):129-34
Categorization of the assay/approach:
Status of the assay:
Categorization according to "exposure duration/effect":
Categorization according to combination of type of effect, endpoint measured and/or method used:
Categorization according to what "R" from the "3R" concept is this assay an alternative:
Relative mRNA expression levels of genes involved in xenobiotic metabolism, thyroid hormon transport and lipid metabolism (mostly cytochrome P4501A)
other references: Hickley et al., Toxicol Sci. 2009 Oct;111(2):311-20, Hervé et al., Toxicol Sci. 2010 Feb;113(2):380-91, ...
Cultured CEH were prepared from day 19 embryos by collagenase digestion and ﬁltration of pooled liver samples (n=50) as described previously (Kennedy et al., 1993; Head et al., 2006).