Bird embryo hepatocyte in vitro assay Back to list of tests

Bird embryo hepatocyte in vitro assay


Effects on mRNA expression in bird (mostly chicken) hepatocytes

Crump et al., Comp Biochem Physiol C Toxicol Pharmacol. 2011 Aug;154(2):129-34

Categorization of the assay/approach:

Status of the assay:

Application domain:

Categorization according to "exposure duration/effect":

Categorization according to combination of type of effect, endpoint measured and/or method used:

Categorization according to what "R" from the "3R" concept is this assay an alternative:


Relative mRNA expression levels of genes involved in xenobiotic metabolism, thyroid hormon transport and lipid metabolism (mostly cytochrome P4501A)

other references: Hickley et al., Toxicol Sci. 2009 Oct;111(2):311-20, Hervé et al., Toxicol Sci. 2010 Feb;113(2):380-91, ...

Cultured CEH were prepared from day 19 embryos by collagenase digestion and filtration of pooled liver samples (n=50) as described previously (Kennedy et al., 1993; Head et al., 2006).