Fish cell - VTG induction Back to list of tests

Fish cell - VTG induction

Description:

The use of primary hepatocytes or specific cell lines (RTH-149 and ZF-L - Christianson-Heiska and Isomaaa 2008) for assessment of estrogenic potencies - induction of vitellogen VTG is measured by various methods.

Reference:
Christianson-Heiska and Isomaaa. 2008 Toxicology in Vitro 22, 3, 589-597 / Björkblom C et al., Chemosphere. 2008 Oct;73(7):1064-70.

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Status of the assay:

Application domain:

Categorization according to "exposure duration/effect":

Categorization according to combination of type of effect, endpoint measured and/or method used:

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Details:

Ohter references:

The male three-spined stickleback (Gasterosteus aculeatus) hepatocyte assay with vitellogenin induction as an endpoint was used for the detection of estrogenic activity in solid phase extracts of wastewater effluents (Björkblom C et al., Chemosphere. 2008 Oct;73(7):1064-70. Epub 2008 Sep 9.)

Induction of VTG in primary hepatocyte cultures from brown trout is measured (Christianson-Heiska and Isomaaa. Toxicology in Vitro, Volume 22, Issue 3, April 2008, Pages 589-597.)

Induction of VTG in primary hepatocytes from common carp (Cyprinus carpio) is measured (Bickley LK et al. Aquat Toxicol. 2009 Sep 14;94(3):195-203. Epub 2009 Jul 16.)

A competitive indirect ELISA was used to determine the level of vtg in primary hepatocytes from common carp (Cyprinus carpio) (Rankouhi TR. et al. Toxicol Sci. 2002 May;67(1):75-80.)

Non-competitive enzyme-linked immunosorbent assay (ELISA) method to investigate estrogenic activities of selected PFCs using vitellogenin (VTG) induction in primary cultured hepatocytes of freshwater male tilapia (Oreochromis niloticus) (Liu et al. Aquat Toxicol. 2007 Dec 30;85(4):267-77. Epub 2007 Sep 26.)

An in vitro assay was developed for measuring Vtg induction, using cultured primary hepatocytes from genetically uniform strains of carp (Cyprinus carpio). Vtg production was measured by indirect competitive ELISA, using a polyclonal antiserum against goldfish Vtg (Smeets et al. Toxicol Appl Pharmacol. 1999 May 15;157(1):68-76.)

 Hollert H. Environ Sci Pollut Res Int. 2005 Nov;12(6):347-60.